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Hoefer te77 semi-transfer unit
Te77 Semi Transfer Unit, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/te77+semi-transfer+unit/semi+dry+transfer+te70x/pmc09966966-59-40-43
Average 90 stars, based on 1 article reviews
te77 semi-transfer unit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Membrane:

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence.
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% (v/v) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% (v/v) Tween-20) plus 5% (w/v) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [26].

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% ( v / v ) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% ( v / v ) Tween-20) plus 5% ( w / v ) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [ ].

Incubation:

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence.
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% (v/v) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% (v/v) Tween-20) plus 5% (w/v) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [26].

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% ( v / v ) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% ( v / v ) Tween-20) plus 5% ( w / v ) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [ ].

Recombinant:

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence.
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% (v/v) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% (v/v) Tween-20) plus 5% (w/v) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [26].

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% ( v / v ) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% ( v / v ) Tween-20) plus 5% ( w / v ) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [ ].

Generated:

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence.
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% (v/v) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% (v/v) Tween-20) plus 5% (w/v) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [26].

Article Title: The Cell Wall Deacetylases Spy1094 and Spy1370 Contribute to Streptococcus pyogenes Virulence
Article Snippet: Whole bacterial cell lysates were separated on a 12.5% polyacrylamide gel and transferred onto a nitrocellulose membrane (Bio Rad) in a transfer buffer (25 mM Tris-HCl, 192 mM glycine, 20% ( v / v ) methanol, pH 8.3) using a TE77 semi-transfer unit (Hoefer, Holliston, MA, USA) at 200 V, 50 mA/gel for 1 h. The membrane was incubated with TBS-T (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 0.1% ( v / v ) Tween-20) plus 5% ( w / v ) skim milk powder at RT for 1 h and then washed once with TBS-T. Antibodies against recombinant forms of Spy1094 and Spy1370 were generated in rabbits as previously described [ ].



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